{"id":33,"date":"2025-06-29T18:07:33","date_gmt":"2025-06-29T18:07:33","guid":{"rendered":"https:\/\/antoniosdougalis.com\/?page_id=33"},"modified":"2025-11-11T18:44:47","modified_gmt":"2025-11-11T18:44:47","slug":"portfolio-work","status":"publish","type":"page","link":"https:\/\/antoniosdougalis.com\/?page_id=33","title":{"rendered":"Portfolio\/Work"},"content":{"rendered":"\t\t
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Published original peer reviewed research manuscripts<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Lukkarinen, H., Luikku, A., Dougalis, A<\/strong>, Pelkonen, A, Gomez-Budia, M., Abushik, P., Novosolova, N., J\u00e4ntti, H., Elomaa, A., Kaipainen, A., Klimko, N, Walle, P., Jyrkk\u00e4nen KH.,\u00a0 Danner, N, Piilonen, <\/sup>K., Firtser, N., Immonen, A., J\u00e4\u00e4skel\u00e4inen, J., Kahle, C., Eide, K., Macosko, E., Stevens, B., Takalo, B., Juopperi, SN., Kemppainen, S., Martiskainen, <\/sup>H., Lipponen, A.,\u00a0 Hiltunen, M., Rauramaa., T, Malm, T., Leinonen, V (2025)<\/strong>. How to obtain a high-quality brain biopsy during shunt surgery for precision diagnostics and multiomic neuroresearch, Brain. In Revision\/Press.<\/strong><\/p>

\u00a0<\/sup>Pelkonen A, Lezhneva V, Ryynanen T, Kreutzer J, Sitnikova V, Gomez-Budia M,Della Pietra A, Dougalis A<\/strong>, Kyyriainen J, Torkamani-Azar M, Eronen H, Rauramaa T, Immonen A, Leinonen V, Sierra A, Kalviainen R,\u00a0 Malm T (2025). <\/strong>Microelectrode array scaled for human hippocampal slices. Biorxiv<\/strong>; DOI:\u00a010.1101\/2025.02.27.640534<\/a><\/p>

Dougalis A<\/strong>*, Abushik P*, Pelkonen A, Guidice L, G\u00f3mez-Budia M, Novosolova N, V\u00e4lim\u00e4ki NN, Rezaie M,\u00a0 Nurkhametova M,\u00a0 Giniatullina R, Shakirzyanova A, Shah A, Rauramaa T, Stevens B,\u00a0 Macosko EZ, Hiltunen M, Leinonen V, Malm T (2025<\/strong>). Early amyloid spine response and impaired synaptic transmission of pyramidal neurons in human biopsies with Alzheimer’s Disease-related pathology. Biorxiv<\/strong>; \u00a0DOI: 10.1101\/2025.01.07.630516<\/a>. * shared first authors and authors for communication.<\/p>

K\u00e4lv\u00e4l\u00e4 S, Abushik P, Dougalis A<\/strong>, Malm T, Pelkonen A, Lehtonen S (2024)<\/strong>. Air-liquid interface culture of midbrain organoids improves neuronal functionality and integration of microglia.\u00a0 Biorxiv<\/strong>; \u00a0DOI: 10.1101\/2023.10.10.561672.<\/p>

Gazestani V, Kamath T, Nadaf NM, Dougalis A<\/strong>, Burris SJ, Rooney B, Junkkari A, Vanderburg C, Pelkonen A, Gomez-Budia M, V\u00e4lim\u00e4ki NN, Rauramaa T, Therrien M, Koivisto AM, Tegtmeyer M, Herukka SK, Abdulraouf A, Marsh SE, Hiltunen M, Nehme R, Malm T, Stevens B, Leinonen V, Macosko EZ (2023)<\/strong>. Early Alzheimer’s disease pathology in human cortex involves transient cell states. Cell<\/strong>; 186(20):4438-4453.e23. doi: 10.1016\/j.cell.2023.08.005. PMID: 37774681.<\/p>

Fagerlund I*, Dougalis A*,<\/strong> Shakirzyanova A, G\u00f3mez-Budia M, Pelkonen A, Konttinen H, Ohtonen S, Fazaludeen MF, Koskuvi M, Kuusisto J, Hern\u00e1ndez D, Pebay A, Koistinaho J, Rauramaa T, Lehtonen \u0160, Korhonen P, Malm T (2021).<\/strong> Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids. Cells<\/strong>; 11(1):124. doi: 10.3390\/cells11010124. PMID: 35011686; PMCID: PMC8750120. * shared first authors<\/p>

Pelkonen A, Pistono C, Klecki P, G\u00f3mez-Budia M, Dougalis A,<\/strong> Konttinen H, Stanov\u00e1 I, Fagerlund I, Leinonen V, Korhonen P, Malm T (2021).<\/strong> Functional Characterization of Human Pluripotent Stem Cell-Derived Models of the Brain with Microelectrode Arrays. Cells<\/strong>; 11(1):106. doi: 10.3390\/cells11010106. PMID: 35011667; PMCID: PMC8750870.<\/p>

Mannal N, Kleiner K, Fauler M, Dougalis A<\/strong>, Poetschke C, Liss B. Multi-Electrode Array Analysis Identifies Complex Dopamine Responses and Glucose Sensing Properties of Substantia Nigra Neurons in Mouse Brain Slices (2021)<\/strong>. Front Synaptic Neurosci<\/strong>; 13:635050. doi: 10.3389\/fnsyn.2021.635050. PMID: 33716704; PMCID: PMC7952765.<\/p>

Ortner, N., Bock, G., Dougalis, A.,<\/strong> Kharitova, M., Duda, J., Tuluc, P., Pomberger, T., Stefanova, N., Pitterl, F.,Ciossek, T., Oberachr, H., Draheims, J, H., Liss, B., Striessnig, J (2017)<\/strong>. \u00a0Lower affinity of isradipine for L-type Ca2+ channels during substantia nigra dopamine neuron-like activity: implications for neuroprotection in Parkinson’s disease. Journal of Neuroscience<\/strong>, doi: 10.1523\/JNEUROSCI.2946-16.2017. PMID:28592699<\/p>

Dougalis, A<\/strong>., Matthews, A.C., Birgit Liss & Ungless, MA. (2017).<\/strong> Ionic currents influencing autorhythmicity and pacemaking in dopamine neurons of the ventrolateral periaquaductal gray and dorsal raphe nucleus (vlPAG\/DR): a voltage-clamp and computational modeling study. Journal of Computational Neuroscience<\/strong>, 42, 275-305, doi: 10.1007\/s10827-017-0641-0. PMID:28367595<\/p>

Poetschke, C., Dragicevic, E., Duda, J., Benkert, J., Dougalis, A<\/strong>., DeZio, R., Snutch, T.P., Striessnig, J. & Liss, B (2015).<\/strong> Compensatory T-type Ca2+ channel activity alters D2-autoreceptor responses of Substantia nigra dopamine neurons from Cav1.3 L-type Ca2+ channel KO mice. Scientific Reports<\/strong>, 5, 13688, doi: 10.1038\/srep13688.PMID:26381090<\/p>

Dougalis, A.,<\/strong> Matthews, AC., Bishop, MW., Brischoux, F., Kobayashi, K., & Ungless, MA. (2012).<\/strong> Functional properties of dopamine neurons and co-expression of vasoactiveintestinal-polypeptide in the dorsal raphe nucleus and ventro-lateral periaquaductal grey. European Journal of Neuroscience<\/strong>, 36, 3322-3332.PMID:22925150<\/p>

Cambray, S., Arber, C., Little, G., Dougalis, A<\/strong>., di Paola, V., Ungless, MA., Li, M., & Rodr\u00edguez, T.A. (2012).<\/strong> Activin induces differentiation and specifies cortical interneuron identity in ESC derived telencephalic neural precursors. Nature Communications<\/strong>, 3, 841, doi:10.1038\/ncomms1817.PMID:22588303<\/p>

Bishop, M.W., Chakraborty, S., Matthews, A.C., Dougalis, A<\/strong>., Wood, N.W., Festenstein, R., Ungless, M.A. (2010).<\/strong> Hyperexcitable dopamine neurons in PINK1 and HrtA2\/Omi deficient mouse models of Parkinson\u2019s disease. Journal of Neurophysiology<\/strong>, 104, 3009-3020. PMID:20926611<\/p>

Dougalis<\/strong>, A. & Lees, G. (2005).<\/strong> The endocannabinoid signaling system: Implications in anaesthesia and the pain clinic. Current Anaesthesia & Critical Care<\/strong>, 16, 71-80.<\/p>

Dougalis, A<\/strong>., Lees, G., Ganellin, C.R. (2004).<\/strong> The sleep lipid oleamide may represent an endogenous anticonvulsant: an in vitro comparative study in the 4-aminopyridine rat brain-slice model. Neuropharmacology<\/strong>, 46, 541-554. PMID:14975678<\/p>

Lees, G. & Dougalis, A<\/strong>. (2004)<\/strong>. Differential effects of the sleep-inducing lipid oleamide and cannabinoids on the induction of long-term potentiation in the CA1 neurons of the rat hippocampus in vitro. Brain Research<\/strong>, 997, 1-14. PMID:14715144<\/p>

Dougalis, A.,<\/strong> Lees, G., Ganellin, C.R. (2004)<\/strong>. The sleep-inducing brain lipid cis-oleamide (cOA) does not modulate serotonergic transmission in the CA1 pyramidal neurons of the hippocampus in vitro. Neuropharmacology<\/strong>, 46, 63-73. PMID:14654098<\/p>

Dougalis Antonios (2005). <\/strong>Cellular actions of putative sleep hormones and depressant drugs in the mammalian brain (PhD Thesis, Library of Royal Pharmaceutical Society of Great Britain).<\/p>

\u00a0<\/strong><\/p>

Work in progress (under review\/revision, as in June 2025)<\/strong><\/p>

Antonios Dougalis<\/strong> *, Anssi Pelkonen, Dilyara Nurkhametova, Christiaan P.J. de Kock, Eline J. Mertens, Huibert D. Mansvelder, Hilde Lavreysen, Nachiket D. Kashikar, Tuomas Rauramaa, Antti Luikku, Ville Leinonen & Tarja Malm 2024 Single-cell and multielectrode array analysis of neuronal and network properties of small volume human frontal cortex biopsies in vitro. Cerebral Cortex<\/strong>.<\/p>

Dougalis A<\/strong>*, Abushik P*, Pelkonen A, Guidice L, G\u00f3mez-Budia M, Novosolova N, V\u00e4lim\u00e4ki NN, Rezaie M,\u00a0 Nurkhametova M,\u00a0 Giniatullina R, Shakirzyanova A, Shah A, Rauramaa T, Stevens B,\u00a0 Macosko EZ, Hiltunen M, Leinonen V, Malm T. Early amyloid spine response and impaired synaptic transmission of pyramidal neurons in human biopsies with Alzheimer’s Disease-related pathology. * shared first authors and authors for communication. Nature Neuroscience<\/strong><\/p>

K\u00e4lv\u00e4l\u00e4 S, Abushik P, Dougalis A<\/strong>, Malm T, Pelkonen A, Lehtonen S. Air-liquid interface culture of midbrain organoids improves neuronal functionality and integration of microglia.\u00a0 J Neuroscience<\/strong><\/p>

Anssi Pelkonen, Vera Lezhneva, Tomi Ryynanen, Joose Kreutzer, Valeriia Sitnikova, Mireia Gomez-Budia, Adriana Della Pietra, Antonios Dougalis<\/strong>, Jenni Kyyriainen, Mastaneh Torkamani-Azar, Henri Eronen, Tuomas Rauramaa, Arto Immonen, Ville Leinonen, Alejandra Sierra, Reetta Kalviainen, Tarja Malm. Biosensors & Bioelectronics<\/strong><\/p>

Lukkarinen, H., Luikku, A., Dougalis, A<\/strong>, Pelkonen, A, Gomez-Budia, M., Abushik, P., Novosolova, N., J\u00e4ntti, H., Elomaa, A., Kaipainen, A., Klimko, N, Walle, P., Jyrkk\u00e4nen KH.,\u00a0 Danner, N, Piilonen, <\/sup>K., Firtser, N., Immonen, A., J\u00e4\u00e4skel\u00e4inen, J., Kahle, C., Eide, K., Macosko, E., Stevens, B., Takalo, B., Juopperi, SN., Kemppainen, S., Martiskainen, <\/sup>H., Lipponen, A.,\u00a0 Hiltunen, M., Rauramaa., T, Malm, T., Leinonen, V (2025)<\/strong>. How to obtain a high-quality brain biopsy during shunt surgery for precision diagnostics and multiomic neuroresearch, Brain.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Published original peer reviewed research manuscripts Orchid Google Scholar \u00a0 Lukkarinen, H., Luikku, A., Dougalis, A, Pelkonen, A, Gomez-Budia, M., Abushik, P., Novosolova, N., J\u00e4ntti, H., Elomaa, A., Kaipainen, A., Klimko, N, Walle, P., Jyrkk\u00e4nen KH.,\u00a0 Danner, N, Piilonen, K., Firtser, N., Immonen, A., J\u00e4\u00e4skel\u00e4inen, J., Kahle, C., Eide, K., Macosko, E., Stevens, B., Takalo, […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","footnotes":""},"class_list":["post-33","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=\/wp\/v2\/pages\/33","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33"}],"version-history":[{"count":368,"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":2356,"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=\/wp\/v2\/pages\/33\/revisions\/2356"}],"wp:attachment":[{"href":"https:\/\/antoniosdougalis.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}