Lukkarinen, H., Luikku, A., Dougalis, A, Pelkonen, A, Gomez-Budia, M., Abushik, P., Novosolova, N., Jäntti, H., Elomaa, A., Kaipainen, A., Klimko, N, Walle, P., Jyrkkänen KH.,  Danner, N, Piilonen^, K., Firtser, N., Immonen, A., Jääskeläinen, J., Kahle, C., Eide, K., Macosko, E., Stevens, B., Takalo, B., Juopperi, SN., Kemppainen, S., Martiskainen^, H., Lipponen, A.,  Hiltunen, M., Rauramaa., T, Malm, T., Leinonen, V (2025). How to obtain a high-quality brain biopsy during shunt surgery for precision diagnostics and multiomic neuroresearch, Brain. In Revision/Press.

 Pelkonen A, Lezhneva V, Ryynanen T, Kreutzer J, Sitnikova V, Gomez-Budia M,Della Pietra A, Dougalis A, Kyyriainen J, Torkamani-Azar M, Eronen H, Rauramaa T, Immonen A, Leinonen V, Sierra A, Kalviainen R,  Malm T (2025). Microelectrode array scaled for human hippocampal slices. Biorxiv; DOI: 10.1101/2025.02.27.640534

Dougalis A*, Abushik P*, Pelkonen A, Guidice L, Gómez-Budia M, Novosolova N, Välimäki NN, Rezaie M,  Nurkhametova M,  Giniatullina R, Shakirzyanova A, Shah A, Rauramaa T, Stevens B,  Macosko EZ, Hiltunen M, Leinonen V, Malm T (2025). Early amyloid spine response and impaired synaptic transmission of pyramidal neurons in human biopsies with Alzheimer’s Disease-related pathology. Biorxiv;  DOI: 10.1101/2025.01.07.630516. * shared first authors and authors for communication.

Kälvälä S, Abushik P, Dougalis A, Malm T, Pelkonen A, Lehtonen S (2024). Air-liquid interface culture of midbrain organoids improves neuronal functionality and integration of microglia.  Biorxiv;  DOI: 10.1101/2023.10.10.561672.

Gazestani V, Kamath T, Nadaf NM, Dougalis A, Burris SJ, Rooney B, Junkkari A, Vanderburg C, Pelkonen A, Gomez-Budia M, Välimäki NN, Rauramaa T, Therrien M, Koivisto AM, Tegtmeyer M, Herukka SK, Abdulraouf A, Marsh SE, Hiltunen M, Nehme R, Malm T, Stevens B, Leinonen V, Macosko EZ (2023). Early Alzheimer’s disease pathology in human cortex involves transient cell states. Cell; 186(20):4438-4453.e23. doi: 10.1016/j.cell.2023.08.005. PMID: 37774681.

Fagerlund I*, Dougalis A*, Shakirzyanova A, Gómez-Budia M, Pelkonen A, Konttinen H, Ohtonen S, Fazaludeen MF, Koskuvi M, Kuusisto J, Hernández D, Pebay A, Koistinaho J, Rauramaa T, Lehtonen Š, Korhonen P, Malm T (2021). Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids. Cells; 11(1):124. doi: 10.3390/cells11010124. PMID: 35011686; PMCID: PMC8750120. * shared first authors

Pelkonen A, Pistono C, Klecki P, Gómez-Budia M, Dougalis A, Konttinen H, Stanová I, Fagerlund I, Leinonen V, Korhonen P, Malm T (2021). Functional Characterization of Human Pluripotent Stem Cell-Derived Models of the Brain with Microelectrode Arrays. Cells; 11(1):106. doi: 10.3390/cells11010106. PMID: 35011667; PMCID: PMC8750870.

Mannal N, Kleiner K, Fauler M, Dougalis A, Poetschke C, Liss B. Multi-Electrode Array Analysis Identifies Complex Dopamine Responses and Glucose Sensing Properties of Substantia Nigra Neurons in Mouse Brain Slices (2021). Front Synaptic Neurosci; 13:635050. doi: 10.3389/fnsyn.2021.635050. PMID: 33716704; PMCID: PMC7952765.

Ortner, N., Bock, G., Dougalis, A., Kharitova, M., Duda, J., Tuluc, P., Pomberger, T., Stefanova, N., Pitterl, F.,Ciossek, T., Oberachr, H., Draheims, J, H., Liss, B., Striessnig, J (2017).  Lower affinity of isradipine for L-type Ca2+ channels during substantia nigra dopamine neuron-like activity: implications for neuroprotection in Parkinson’s disease. Journal of Neuroscience, doi: 10.1523/JNEUROSCI.2946-16.2017. PMID:28592699

Dougalis, A., Matthews, A.C., Birgit Liss & Ungless, MA. (2017). Ionic currents influencing autorhythmicity and pacemaking in dopamine neurons of the ventrolateral periaquaductal gray and dorsal raphe nucleus (vlPAG/DR): a voltage-clamp and computational modeling study. Journal of Computational Neuroscience, 42, 275-305, doi: 10.1007/s10827-017-0641-0. PMID:28367595

Poetschke, C., Dragicevic, E., Duda, J., Benkert, J., Dougalis, A., DeZio, R., Snutch, T.P., Striessnig, J. & Liss, B (2015). Compensatory T-type Ca2+ channel activity alters D2-autoreceptor responses of Substantia nigra dopamine neurons from Cav1.3 L-type Ca2+ channel KO mice. Scientific Reports, 5, 13688, doi: 10.1038/srep13688.PMID:26381090

Dougalis, A., Matthews, AC., Bishop, MW., Brischoux, F., Kobayashi, K., & Ungless, MA. (2012). Functional properties of dopamine neurons and co-expression of vasoactiveintestinal-polypeptide in the dorsal raphe nucleus and ventro-lateral periaquaductal grey. European Journal of Neuroscience, 36, 3322-3332.PMID:22925150

Cambray, S., Arber, C., Little, G., Dougalis, A., di Paola, V., Ungless, MA., Li, M., & Rodríguez, T.A. (2012). Activin induces differentiation and specifies cortical interneuron identity in ESC derived telencephalic neural precursors. Nature Communications, 3, 841, doi:10.1038/ncomms1817.PMID:22588303

Bishop, M.W., Chakraborty, S., Matthews, A.C., Dougalis, A., Wood, N.W., Festenstein, R., Ungless, M.A. (2010). Hyperexcitable dopamine neurons in PINK1 and HrtA2/Omi deficient mouse models of Parkinson’s disease. Journal of Neurophysiology, 104, 3009-3020. PMID:20926611

Dougalis, A. & Lees, G. (2005). The endocannabinoid signaling system: Implications in anaesthesia and the pain clinic. Current Anaesthesia & Critical Care, 16, 71-80.

Dougalis, A., Lees, G., Ganellin, C.R. (2004). The sleep lipid oleamide may represent an endogenous anticonvulsant: an in vitro comparative study in the 4-aminopyridine rat brain-slice model. Neuropharmacology, 46, 541-554. PMID:14975678

Lees, G. & Dougalis, A. (2004). Differential effects of the sleep-inducing lipid oleamide and cannabinoids on the induction of long-term potentiation in the CA1 neurons of the rat hippocampus in vitro. Brain Research, 997, 1-14. PMID:14715144

Dougalis, A., Lees, G., Ganellin, C.R. (2004). The sleep-inducing brain lipid cis-oleamide (cOA) does not modulate serotonergic transmission in the CA1 pyramidal neurons of the hippocampus in vitro. Neuropharmacology, 46, 63-73. PMID:14654098

Dougalis Antonios (2005). Cellular actions of putative sleep hormones and depressant drugs in the mammalian brain (PhD Thesis, Library of Royal Pharmaceutical Society of Great Britain).

Work in progress (under review/revision, as in June 2025)

Antonios Dougalis *, Anssi Pelkonen, Dilyara Nurkhametova, Christiaan P.J. de Kock, Eline J. Mertens, Huibert D. Mansvelder, Hilde Lavreysen, Nachiket D. Kashikar, Tuomas Rauramaa, Antti Luikku, Ville Leinonen & Tarja Malm 2024 Single-cell and multielectrode array analysis of neuronal and network properties of small volume human frontal cortex biopsies in vitro. Cerebral Cortex.

Dougalis A*, Abushik P*, Pelkonen A, Guidice L, Gómez-Budia M, Novosolova N, Välimäki NN, Rezaie M,  Nurkhametova M,  Giniatullina R, Shakirzyanova A, Shah A, Rauramaa T, Stevens B,  Macosko EZ, Hiltunen M, Leinonen V, Malm T. Early amyloid spine response and impaired synaptic transmission of pyramidal neurons in human biopsies with Alzheimer’s Disease-related pathology. * shared first authors and authors for communication. Nature Neuroscience

Kälvälä S, Abushik P, Dougalis A, Malm T, Pelkonen A, Lehtonen S. Air-liquid interface culture of midbrain organoids improves neuronal functionality and integration of microglia.  J Neuroscience

Anssi Pelkonen, Vera Lezhneva, Tomi Ryynanen, Joose Kreutzer, Valeriia Sitnikova, Mireia Gomez-Budia, Adriana Della Pietra, Antonios Dougalis, Jenni Kyyriainen, Mastaneh Torkamani-Azar, Henri Eronen, Tuomas Rauramaa, Arto Immonen, Ville Leinonen, Alejandra Sierra, Reetta Kalviainen, Tarja Malm. Biosensors & Bioelectronics

Lukkarinen, H., Luikku, A., Dougalis, A, Pelkonen, A, Gomez-Budia, M., Abushik, P., Novosolova, N., Jäntti, H., Elomaa, A., Kaipainen, A., Klimko, N, Walle, P., Jyrkkänen KH.,  Danner, N, Piilonen^, K., Firtser, N., Immonen, A., Jääskeläinen, J., Kahle, C., Eide, K., Macosko, E., Stevens, B., Takalo, B., Juopperi, SN., Kemppainen, S., Martiskainen^, H., Lipponen, A.,  Hiltunen, M., Rauramaa., T, Malm, T., Leinonen, V (2025). How to obtain a high-quality brain biopsy during shunt surgery for precision diagnostics and multiomic neuroresearch, Brain.